Identification of rat serum alkaline phosphatase isoenzyme by means of wheat germ agglutinin.

نویسندگان

  • H Wada
  • N Niwa
  • T Hayakawa
  • H Tsuge
چکیده

Wheat germ agglutinin (WGA) precipitates bone type serum alkaline phosphatase (sALP) isoenzyme specifically. The precipitates are composed of the macromolecules of WGA and "bone type sALP" (WGA-ALP complex). In order to use bone type sALP as a marker in polyacrylamide gel electrophoresis (PAGE), a method to separate "bone type sALP" from the "WGA-ALP complex" was established by using N-acetyl-D-glucosamine (GlcNAc)-Sepharose 6E column chromatography. It was concluded that this method is useful for clinical examination in the rat.

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عنوان ژورنال:
  • Experimental animals

دوره 46 1  شماره 

صفحات  -

تاریخ انتشار 1997